aldosterone plasma levels  (Tecan Systems)

Journal: Metabolism: clinical and experimental

Article Title: Caloric restriction improves glucose homeostasis, yet increases cardiometabolic risk in caveolin-1-deficient mice

doi: 10.1016/j.metabol.2018.01.012

Figure Lengend Snippet: Baseline characteristics of WT vs. CAV-1 KO mice

Article Snippet: Plasma aldosterone and corticosterone levels were determined by RIA as per manufacturer’s instructions (Coat-A-Count, Siemens, Los Angeles, CA).

Techniques:

Journal: Metabolism: clinical and experimental

Article Title: Caloric restriction improves glucose homeostasis, yet increases cardiometabolic risk in caveolin-1-deficient mice

doi: 10.1016/j.metabol.2018.01.012

Figure Lengend Snippet: Circulating aldosterone (A), PRA (B), aldosterone to PRA ratio (C), corticosterone (E), and aldosterone to corticosterone ratio (F) in WT and CAV-1 KO maintained on ad lib vs. CR diet. Aldosterone release in response to 10−8 M Ang II, 8.7 mM K+ and 10−10 M ACTH (D) was measured in Zona Glomerulosa cells acutely isolated from mice in the four experimental groups. *p < 0.05, ** p < 0.005 vs. WT on the same diet; # p < 0.05 vs. the same genotype on ad lib diet.

Article Snippet: Plasma aldosterone and corticosterone levels were determined by RIA as per manufacturer’s instructions (Coat-A-Count, Siemens, Los Angeles, CA).

Techniques: Isolation

Journal: Journal of Innate Immunity

Article Title: GDF15 Suppresses Lymphoproliferation and Humoral Autoimmunity in a Murine Model of Systemic Lupus Erythematosus

doi: 10.1159/000523991

Figure Lengend Snippet: GDF15 negatively regulates TLR-9 dependent inflammation and cell proliferation. a C57BL/6 ( n = 7), Gdf15−/− ( n = 8) , Gdf15−/− lpr/lpr ( n = 10) and control C57BL/6 lpr/lpr ( n = 7) female mice were bled at month 6 to determine serum levels of corticosterone and aldosterone. Data represent means ± SD. b Expression levels of Tlr7 and Tlr-9 in spleens of 6-month-old C57BL/6 ( n = 5), Gdf15−/− ( n = 5) , Gdf15−/− lpr/lpr ( n = 6), and control C57BL/6 lpr/lpr ( n = 6) females were quantified by real-time PCR. Data are shown as means ± SD of the ratio of the specific mRNA versus that of GAPDH mRNA, * p < 0.05. c The production of GDF15 was quantified in supernatants of BM derived macrophage isolated from C57BL/6 ( n = 7) and C57BL/6 lpr/lpr ( n = 12) mice by ELISA 24 h upon CpG 1668 (100 ng/mL) stimulation. Data represent means ± SD; *** p < 0.001. d The proliferative activity of spleen lymphocytes isolated from 8-weeks old mice, stimulated with 10% FCS ( n = 4) or CpG ( n = 8) was evaluated by MTT assay 72 h upon CpG 1668 (50 ng/mL). The graphs represent a representative experiment (from three independent experiments) performed in a single run; presented as mean ± SD; * p < 0.05. e Expression analysis of preselected interferon type I dependent genes in BM-derived macrophages isolated from all four strains of mice and stimulated with LPS (10 ng/mL), CpG 1668 (100 ng/mL), and Imiquimod (1 μg/mL); n = 5 per each group and genotype. The expression of selected genes was determined in BM-derived macrophages 4 h poststimulation. The graphs represent a representative experiment (from three independent experiments) performed in a single run. Data represent means ± SD; * p < 0.05; ** p < 0.01, *** p < 0.001. f The differences in TNFα expression ( n = 4 per genotype and stimulation) and IL12 and TNFα production upon CpG 1668 (100 ng/mL) stimulation (or costimulation with 20 ng/mL of IFNγ) in BM-derived macrophages from C57BL/6, Gdf15−/− as well as Gdf15−/− lpr/lpr and control C57BL/6 lpr/lpr mice were evaluated with qPCR (4 h) and ELISA (24 h). The biological replicates of n = 5 per group were used. Data represent means ± SD; * p < 0.05; ** p < 0.01. The graphs represent a representative experiment (from three independent experiments) performed in a single run.

Article Snippet: Plasma aldosterone levels were analysed using an enzyme-linked immunosorbent assay (ELISA, RE52301, IBL International GmbH, Hamburg, Germany).

Techniques: Control, Expressing, Real-time Polymerase Chain Reaction, Derivative Assay, Isolation, Enzyme-linked Immunosorbent Assay, Activity Assay, MTT Assay

Journal: Kidney Medicine

Article Title: Renin-Angiotensin-Aldosterone System Activation and Diuretic Response in Ambulatory Patients With Heart Failure

doi: 10.1016/j.xkme.2022.100465

Figure Lengend Snippet: Flow chart depicting the sampling of initial YTCC cohort to produce final analytic sample. Abbreviations: FENa-DE, fractional excretion of sodium at peak diuresis per 2-fold higher diuretic dose; Na-DE; total sodium excreted per 2-fold higher diuretic dose; RAAS, renin-angiotensin-aldosterone system; YTCC, Yale Transitional Care Center.

Article Snippet: The plasma aldosterone level was measured by radioimmunoassay kit (Alpco) as per manufacturer’s instructions.

Techniques: Sampling

Journal: Kidney Medicine

Article Title: Renin-Angiotensin-Aldosterone System Activation and Diuretic Response in Ambulatory Patients With Heart Failure

doi: 10.1016/j.xkme.2022.100465

Figure Lengend Snippet: Associations of Urine and Plasma RAAS Measures With Diuretic Efficiency After Torsemide Administration

Article Snippet: The plasma aldosterone level was measured by radioimmunoassay kit (Alpco) as per manufacturer’s instructions.

Techniques: Clinical Proteomics